TAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Rezensionen (1)

Kat-Nr. : 11114-1-AP

Synonyme

RING4, PSF-1, PSF1, EC:7.4.2.14, ATP-binding cassette sub-family B member 2

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Galerie der Validierungsdaten

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  WB analysis using 11114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11114-1-AP

  WB result of TAP1 antibody (11114-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAP1 transfected HeLa cells.

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  WB analysis using 11114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11114-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11114-1-AP

  IP result of anti-TAP1 (IP:11114-1-AP, 4ug; Detection:11114-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human pancreas cancer using 11114-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11114-1-AP (TAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 11114-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11114-1-AP (TAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11114-1-AP

  Immunofluorescent analysis of HepG2 cells, using TAP1 antibody 11114-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 11114-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11114-1-AP (TAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, Mausmilzgewebe, Maus-Skelettmuskelgewebe, SW480-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

11114-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA TAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAP1 fusion protein Ag1619
• Vollständiger Name: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 70-81 kDa
• GenBank-Zugangsnummer: BC014081
• Gene symbol: TAP1
• Gene ID (NCBI): 6890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.

Publikationen

Species	Application	Title
human	WB	Gastroenterology; microRNA 125a Regulates MHC-I Expression on Esophageal Adenocarcinoma Cells, Associated With Suppression of Antitumor Immune Response and Poor Outcomes of Patients.; Authors - Luigi Mari
human	IHC	Nat Commun; Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes.; Authors - Karama Asleh
mouse	WB	Theranostics; Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells.; Authors - Jingyao Tu
human	IHC	Int J Mol Sci; Antigen Peptide Transporter 1 (TAP1) Promotes Resistance to MEK Inhibitors in Pancreatic Cancers.; Authors - Boya Li
human	WB	J Immunol; Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.; Authors - Zhangchuan Xia ; KD Validated
human	WB,IF	J Biol Chem; The unfolded protein response (UPR)-activated transcription factor X-box-binding protein 1 (XBP1) induces microRNA-346 expression that targets the human antigen peptide transporter 1 (TAP1) mRNA and governs immune regulatory genes.; Authors - Bartoszewski Rafal R

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Adrianna (Verified Customer) (10-24-2022)

This antibody is excellent. I've tried anti TAP1 antibodies from different companies and they all showed brighter bands for contaminants than the TAP1 protein, here I can clearly see my protein of interest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7