TAF1-Specific Polyklonaler Antikörper

TAF1-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20260-1-AP

Synonyme

BA2R, CCG1, CCGS, Cell cycle gene 1 protein, DYT3, KAT4, N TAF1, NSCL2, OF, P250, TAF(II)250, TAF1, TAF1-Specific, TAF2A, TAFII 250, TAFII250, TBP associated factor 250 kDa

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  WB analysis using 20260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20260-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20260-1-AP bindet in WB, IHC, ELISA TAF1-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
• Berechnetes Molekulargewicht: 213 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa, 50 kDa
• GenBank-Zugangsnummer: NM_138923
• Gene symbol: TAF1
• Gene ID (NCBI): 6872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAF1, also named as BA2R, CCG1, CCGS, TAF2A, p250, Cell cycle gene 1 protein, TAFII250 and DYT3, belongs to the TAF1 family. TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex. It contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. TAF1 phosphorylates GTF2A1 and GTF2F1 on Ser residues. It possesses DNA-binding activity. It is essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3) which also called X-linked dystonia-parkinsonism (XDP). The antibody is specific to TAF1. It has several isoforms with predicted MW's of 200-250 kDa and 170-180 kDa, it also could get about 50 kDa band which is caused by the lysate preparation.

Publikationen

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
human	WB	J Clin Invest; Long noncoding RNA HITT coordinates with RGS2 to inhibit PD-L1 translation in T cell immunity; Authors - Qingyu Lin
		J Transl Med; Synergistic effect of mesenchymal stem cell-derived extracellular vesicle and miR-137 alleviates autism-like behaviors by modulating the NF-κB pathway; Authors - Qian Qin