TADA3L Polyklonaler Antikörper

TADA3L Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10839-1-AP

Synonyme

ADA3, ADA3 homolog, ADA3 like protein, hADA3, STAF54, TADA3, TADA3L, Transcriptional adapter 3, Transcriptional adapter 3 like

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Galerie der Validierungsdaten

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  WB analysis using 10839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10839-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 10839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10839-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10839-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10839-1-AP (TADA3L antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:9000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10839-1-AP bindet in WB, IHC, ELISA TADA3L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TADA3L fusion protein Ag1276
• Vollständiger Name: transcriptional adaptor 3 (NGG1 homolog, yeast)-like
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC009240
• Gene symbol: TADA3L
• Gene ID (NCBI): 10474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TADA3 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A)-containing (ATAC) complex and the SPT3 (SUPT3H)-TAF9-GCN5 (KAT2A)/PCAF (KAT2B) acetylase (STAGA) complex. The ATAC complex is a complex with histone acetyltransferase activity on histones H3 and H4, and the PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. TADA3 is also known as a coactivator for p53/TP53-dependent transcriptional activation.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation; Authors - Siyuan Sun