SYNCRIP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SYNCRIP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14024-1-AP

Synonyme

HNRPQ1, HNRPQ, hnRNP Q, GRY-RBP, GRY RBP

---

Galerie der Validierungsdaten

• 

  WB analysis of A431 using 14024-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14024-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14024-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14024-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 14024-1-AP

  IP result of anti-SYNCRIP (IP:14024-1-AP, 4ug; Detection:14024-1-AP 1:500) with A431 cells lysate 1200ug.

• 

  IHC staining of human colon cancer using 14024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14024-1-AP (SYNCRIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14024-1-AP (SYNCRIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 14024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SYNCRIP antibody (14024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

14024-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SYNCRIP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYNCRIP fusion protein Ag5167
• Vollständiger Name: synaptotagmin binding, cytoplasmic RNA interacting protein
• Berechnetes Molekulargewicht: 623 aa, 70 kDa
• Beobachtetes Molekulargewicht: 64-74 kDa
• GenBank-Zugangsnummer: BC032643
• Gene symbol: SYNCRIP
• Gene ID (NCBI): 10492
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptotagmin-binding, cytoplasmic RNA-interacting protein(SYNCRIP), also identified as hnRNP Q, involves in mRNA processing mechanisms. It's component of the CRD-mediated complex that promotes MYC mRNA stability. Also it is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. As previously reported, the 65 and 74 kDa proteins corresponded to two isoforms of SYNCRIP produced from alternatively spliced mRNAs (PMID: 15340051).

Publikationen

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
mouse	WB	Sci Rep; Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.; Authors - Silvia Parisi ; KD Validated