SUPV3L1 Polyklonaler Antikörper

SUPV3L1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12826-1-AP

Synonyme

SUV3-like protein 1, SUV3 like protein 1, SUV3, Suppressor of var1 3-like protein 1, EC:3.6.4.13

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Galerie der Validierungsdaten

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  WB analysis using 12826-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12826-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12826-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 12826-1-AP

  HeLa cell were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:500.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 12826-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12826-1-AP bindet in WB, IF/ICC, IP, ELISA SUPV3L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUPV3L1 fusion protein Ag3542
• Vollständiger Name: suppressor of var1, 3-like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 786 aa, 88 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC036112
• Gene symbol: SUPV3L1
• Gene ID (NCBI): 6832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).

Publikationen

Species	Application	Title
human	WB	J Inherit Metab Dis; Mitochondrial RNA processing defect caused by a SUPV3L1 mutation in two siblings with a novel neurodegenerative syndrome.; Authors - Selma L van Esveld
human	WB	Biochimie; Cathepsin D inhibition during neuronal differentiation selectively affects individual proteins instead of overall protein turnover; Authors - Johannes Schneider