SUMO2/3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SUMO2/3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10947-1-AP

Synonyme

SUMO2, SMT3B, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10947-1-AP

  WB result of SUMO2/3 antibody (10947-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10947-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10947-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10947-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 10947-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10947-1-AP (SUMO2/3 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 10947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (10947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, Jurkat-Zellen, Rekombinantes Protein Protein
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

10947-1-AP bindet in WB, IHC, IF/ICC, ELISA SUMO2/3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUMO2/3 fusion protein Ag1388
• Vollständiger Name: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC008450
• Gene symbol: SUMO2
• Gene ID (NCBI): 6613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Publikationen

Species	Application	Title
human	WB,IF	Cell Death Discov; Loss of PML nuclear bodies in familial amyotrophic lateral sclerosis-frontotemporal dementia; Authors - Francesco Antoniani