SUMO1 Polyklonaler Antikörper

SUMO1 Polyklonal Antikörper für FC, IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10329

Synonyme

DAP 1, GAP modifying protein 1, GMP1, OFC10, PIC1, SENP2, Sentrin, SMT3, SMT3 homolog 3, SMT3C, SMT3H3, SUMO 1, SUMO1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, UBL1

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Galerie der Validierungsdaten

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  IF Staining of A549 using CL488-10329

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 SUMO1 antibody (CL488-10329) at dilution of 1:400.

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  FC experiment of A549 using CL488-10329

  1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SUMO1 (CL488-10329) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A549-Zellen
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10329 bindet in IF, FC SUMO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUMO1 fusion protein Ag0414
• Vollständiger Name: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa, 80-90 kDa
• GenBank-Zugangsnummer: BC006462
• Gene symbol: SUMO1
• Gene ID (NCBI): 7341
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).

1. What is the molecular weight of SUMO1?

The molecular weight of SUMO1 is 12 kDa.

2. What is the cellular localization of SUMO1?

SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).

3. What modifications is SUMO1 subjected to?

SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).

4. What is the function of SUMO1?

SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.

5. What is SUMO1's involvement in disease?

Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).