SUMO1 Polyklonaler Antikörper
SUMO1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10329-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A549-Zellen, HeLa-Zellen, NIH/3T3-Zellen, PC-12-Zellen |
| Erfolgreiche IP | HeLa-Zellen |
| Erfolgreiche Detektion in IHC | humanes Hodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | A549-Zellen |
| Erfolgreiche Detektion in FC (Intra) | A549-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 18 publications below |
| IF | See 2 publications below |
| IP | See 5 publications below |
| CoIP | See 1 publications below |
Produktinformation
10329-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA SUMO1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | SUMO1 fusion protein Ag0414 |
| Vollständiger Name | SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) |
| Berechnetes Molekulargewicht | 12 kDa |
| Beobachtetes Molekulargewicht | 10-12 kDa, 80-90 kDa |
| GenBank-Zugangsnummer | BC006462 |
| Gene symbol | SUMO1 |
| Gene ID (NCBI) | 7341 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).
1. What is the molecular weight of SUMO1?
The molecular weight of SUMO1 is 12 kDa.
2. What is the cellular localization of SUMO1?
SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).
3. What modifications is SUMO1 subjected to?
SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).
4. What is the function of SUMO1?
SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.
5. What is SUMO1's involvement in disease?
Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for SUMO1 antibody 10329-1-AP | Protokoll herunterladen |
| IHC protocol for SUMO1 antibody 10329-1-AP | Protokoll herunterladen |
| IF protocol for SUMO1 antibody 10329-1-AP | Protokoll herunterladen |
| IP protocol for SUMO1 antibody 10329-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Sci Adv XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility | ||
Int J Biol Macromol SUMOylation of GMFB regulates its stability and function in retinal pigment epithelial cells under hyperglycemia | ||
Theranostics Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation. | ||
Free Radic Biol Med Sentrin-specific protease 1 maintains mitochondrial homeostasis through targeting the deSUMOylation of sirtuin-3 to alleviate oxidative damage induced by hepatic ischemia/reperfusion | ||
Anal Chem Sensitive detection of intracellular sumoylation via SNAP tag-mediated translation and RNA polymerase-based amplification. | ||
mSystems Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Zoe (Verified Customer) (10-08-2021) | Hadn’t previously had much luck with antibodies on my Tricky construct so was great to use this one!
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