SULT1E1 Polyklonaler Antikörper

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• KD/KO Validated

SULT1E1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 12522-1-AP

Synonyme

EST, EST 1, Estrogen sulfotransferase, ST1E1, STE, Sulfotransferase 1E1, SULT1E1

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Galerie der Validierungsdaten

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  WB analysis of human lung using 12522-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12522-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 12522-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12522-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12522-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12522-1-AP

  IP result of anti-SULT1E1 (IP:12522-1-AP, 4ug; Detection:12522-1-AP 1:400) with mouse liver tissue lysate 4000ug.

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  IHC staining of human breast cancer using 12522-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12522-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lungengewebe, humanes Lebergewebe, humanes Nebennierengewebe, humanes Nierengewebe, Mausnierengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

12522-1-AP bindet in WB, IP, IF, IHC, ELISA SULT1E1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SULT1E1 fusion protein Ag3216
• Vollständiger Name: sulfotransferase family 1E, estrogen-preferring, member 1
• Berechnetes Molekulargewicht: 294 aa, 35 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC027956
• Gene symbol: SULT1E1
• Gene ID (NCBI): 6783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Oestrogen sulfotransferase ablation sensitizes mice to sepsis.; Authors - Xiaojuan Chai
	IHC	J Am Soc Nephrol; Inhibition of Estrogen Sulfotransferase (SULT1E1/EST) Ameliorates Ischemic Acute Kidney Injury in Mice.; Authors - Anne C Silva Barbosa
human	WB	Biochem Pharmacol; PXR phosphorylated at Ser350 transduces a glucose signal to repress the estrogen sulfotransferase gene in human liver cells and fasting signal in mouse livers.; Authors - Hao Hu
mouse	WB	J Biol Chem; Estrogen sulfotransferase is an oxidative stress responsive gene that gender-specifically affects liver ischemia/reperfusion injury.; Authors - Yan Guo ; KO Validated
human	WB	Biochem Pharmacol; Farnesoid X receptor regulates SULT1E1 expression through inhibition of PGC1α binding to HNF4α.; Authors - Shuai Wang
mouse	WB	Endocrinology; PMID: 28938414; Authors - Wojciech G Garbacz