SULT1A4 Polyklonaler Antikörper

SULT1A4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11376-1-AP

Synonyme

Monoamine-sulfating phenol sulfotransferase, M PST, HAST3, EC:2.8.2.1, Catecholamine-sulfating phenol sulfotransferase

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Galerie der Validierungsdaten

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  WB analysis of human colon using 11376-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human pancreas using 11376-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11376-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11376-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11376-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11376-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11376-1-AP (SULT1A4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11376-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11376-1-AP (SULT1A4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11376-1-AP

  Immunofluorescent analysis of HepG2 cells using 11376-1-AP (SULT1A4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11376-1-AP

  Immunofluorescent analysis of HepG2 cells, using SULT1A4 antibody 11376-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, BxPC-3-Zellen, HepG2-Zellen, humanes Pankreasgewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11376-1-AP bindet in WB, IHC, IF/ICC, ELISA SULT1A4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SULT1A4 fusion protein Ag1935
• Vollständiger Name: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 4
• Berechnetes Molekulargewicht: 295 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC014471
• Gene symbol: SULT1A4
• Gene ID (NCBI): 445329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.