SUCLA2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SUCLA2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 12627-1-AP

Synonyme

SCS-betaA, SCS betaA, EC:6.2.1.5, ATP-specific succinyl-CoA synthetase subunit beta, A-SCS

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12627-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12627-1-AP (SUCLA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12627-1-AP

  WB result of SUCLA2 antibody (12627-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUCLA2 transfected HepG2 cells.

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  WB analysis of L02 using 12627-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12627-1-AP (SUCLA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12627-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12627-1-AP (SUCLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12627-1-AP

  IP result of anti-SUCLA2 (IP:12627-1-AP, 4ug; Detection:12627-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12627-1-AP (SUCLA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12627-1-AP (SUCLA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12627-1-AP (SUCLA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12627-1-AP (SUCLA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12627-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUCLA2 antibody (12627-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, L02-Zellen, Maushirngewebe, Mauslebergewebe, PC-3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

12627-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SUCLA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUCLA2 fusion protein Ag3319
• Vollständiger Name: succinate-CoA ligase, ADP-forming, beta subunit
• Berechnetes Molekulargewicht: 463 aa, 50 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC027587
• Gene symbol: SUCLA2
• Gene ID (NCBI): 8803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUCLA2, β subunit of succinate-CoA ligase, contributes to energy production in mitochondria through the succinate-CoA ligase enzyme. Succinate-CoA ligase catalyses the reversible conversion of succinyl-CoA and ADP or GDP to succinate and ATP or GTP. SUCLA2 mutations cause global protein succinylation and mitochondrial DNA depletion (PMID: 33230181, PMID: 20301762). Mutations in SUCLA2 are also associated with SUCLA2-related mitochondrial DNA depletion syndrome, leigh syndrome, down syndrome (PMID: 23010432).

Publikationen

Species	Application	Title
human	WB	Mol Cell; Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation; Authors - Sophie Trefely
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
human	WB,IHC	Oncogene; Pharmacologically targetable vulnerability in prostate cancer carrying RB1-SUCLA2 deletion.; Authors - Susumu Kohno ; KO Validated
human,mouse	WB,IHC	Oncogene; MUC1 triggers lineage plasticity of Her2 positive mammary tumors; Authors - Zhi Pang
human	IF	Brain Struct Funct; Exclusive neuronal expression of SUCLA2 in the human brain.; Authors - Dobolyi Arpád A