SUB1 Polyklonaler Antikörper

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• KD/KO Validated

SUB1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11956-1-AP

Synonyme

RPO2TC1, Positive cofactor 4, PC4, P15, p14

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Galerie der Validierungsdaten

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  WB analysis of rat liver using 11956-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11956-1-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11956-1-AP

  WB result of SUB1 antibody (11956-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected HeLa cells.

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  WB analysis of HeLa using 11956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11956-1-AP (SUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 11956-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 11956-1-AP (SUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11956-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11956-1-AP (SUB1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11956-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11956-1-AP (SUB1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11956-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11956-1-AP (SUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, C2C12-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11956-1-AP bindet in WB, IHC, IF/ICC, ELISA SUB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUB1 fusion protein Ag2559
• Vollständiger Name: SUB1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 18-23 kDa
• GenBank-Zugangsnummer: BC022339
• Gene symbol: SUB1
• Gene ID (NCBI): 10923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. Catalog#11956-1-AP is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).