Syntaxin 7 Polyklonaler Antikörper

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• KD/KO Validated

Syntaxin 7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 12322-1-AP

Synonyme

Syntaxin-7, STX7

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Galerie der Validierungsdaten

• 

  WB analysis of MDCK cells using 12322-1-AP

  siRNA result of 12322-1-AP (anti-STX7) by Dr.Kazunari Yamashita.

• 

  WB analysis of mouse spleen using 12322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12322-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12322-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 12322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12322-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12322-1-AP

  IP result of anti-Syntaxin 7 (IP:12322-1-AP, 3ug; Detection:12322-1-AP 1:800) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human medulloblastoma using 12322-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human medulloblastoma using 12322-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 12322-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Syntaxin 7 antibody (12322-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, MDCK-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Medulloblastom-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

12322-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Syntaxin 7 und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntaxin 7 fusion protein Ag2975
• Vollständiger Name: syntaxin 7
• Berechnetes Molekulargewicht: 261 aa, 30 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC011975
• Gene symbol: Syntaxin 7
• Gene ID (NCBI): 8417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A; Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.; Authors - Bin Na-Ryum NR
rat	WB,CoIP	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li ; KD Validated
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik
human	WB	Cell Logist; Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins.; Authors - Carolina G Ortiz-Sandoval
human,mouse	WB,IF	Neurobiol Dis; Syntaxin 7 modulates seizure activity in epilepsy; Authors - Junhong Wu
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christian (Verified Customer) (10-12-2020)

The signal is strong and clean

• Applications: Western Blot
• Cell Tissue Type: b16f10