Syntaxin 6 Polyklonaler Antikörper

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• KD/KO Validated

Syntaxin 6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 10841-1-AP

Synonyme

Syntaxin-6, STX6

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Galerie der Validierungsdaten

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  WB analysis of A549 using 10841-1-AP

  WB result of Syntaxin 6 antibody (10841-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Syntaxin 6 transfected A549 cells.

• 

  WB analysis using 10841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10841-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10841-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10841-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IP experiment of mouse brain using 10841-1-AP

  IP result of anti-Syntaxin 6 (IP:10841-1-AP, 3ug; Detection:10841-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

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  IHC staining of mouse brain using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of murine BM-DC using 10841-1-AP

  IF result of anti-STX6 (10841-1-AP) in Murine BM-DC by Dr. LOREDANA SAVEANU.

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  IF Staining of murine Spleen-DC using 10841-1-AP

  IF result of anti-STX6 (10841-1-AP) in Murine Spleen-DC by Dr. LOREDANA SAVEANU.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, A549-Zellen, humanes Hirngewebe, Maushirngewebe, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen, murine BM-DC, murine Spleen-DC

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 11 publications below
• FC: See 1 publications below

Produktinformation

10841-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Syntaxin 6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntaxin 6 fusion protein Ag1284
• Vollständiger Name: syntaxin 6
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC009944
• Gene symbol: Syntaxin 6
• Gene ID (NCBI): 10228
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Publikationen

Species	Application	Title
mouse	IF	Science; IRAP identifies an endosomal compartment required for MHC class I cross-presentation.; Authors - Loredana Saveanu
mouse	IF	Nat Immunol; IRAP(+) endosomes restrict TLR9 activation and signaling.; Authors - Joel Babdor
human	WB	Cell Rep Med; Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis.; Authors - Yamina Hamel
	IF,FC	Nat Commun; IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses.; Authors - Irini Evnouchidou
mouse	IF	Cell Rep; Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation.; Authors - Mirjana Weimershaus
mouse	IF	J Immunol; Conventional dendritic cells require IRAP-Rab14 endosomes for efficient cross-presentation.; Authors - Weimershaus Mirjana M