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STOML2 Polyklonaler Antikörper
STOML2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10348-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Maushirngewebe, C6-Zellen, HEK-293-Zellen, Rattenhirngewebe |
Erfolgreiche IP | Maushirngewebe |
Erfolgreiche Detektion in IHC | humanes Magenkrebsgewebe, humanes Endometriumkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:250-1:1000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 7 publications below |
WB | See 39 publications below |
IHC | See 24 publications below |
IF | See 6 publications below |
IP | See 2 publications below |
Produktinformation
10348-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA STOML2 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | STOML2 fusion protein Ag0363 |
Vollständiger Name | stomatin (EPB72)-like 2 |
Berechnetes Molekulargewicht | 356 aa, 39 kDa |
Beobachtetes Molekulargewicht | 39 kDa |
GenBank-Zugangsnummer | BC002442 |
Gene symbol | STOML2 |
Gene ID (NCBI) | 30968 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for STOML2 antibody 10348-1-AP | Protokoll herunterladen |
IHC protocol for STOML2 antibody 10348-1-AP | Protokoll herunterladen |
IF protocol for STOML2 antibody 10348-1-AP | Protokoll herunterladen |
IP protocol for STOML2 antibody 10348-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Cell Metab DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. | ||
Clin Cancer Res Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma. | ||
EMBO Rep The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L. | ||
Hum Mol Genet Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. | ||
J Pathol Knockdown of stomatin-like protein 2 (STOML2) reduces the invasive ability of glioma cells through inhibition of the NF-κB/MMP-9 pathway. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Gaurav (Verified Customer) (02-15-2024) | Good antibody which works as per the description. Detects the endogenous proteins without any problem.
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FH Hussein (Verified Customer) (03-23-2021) | I validated the antibody specificity using different siRNAs. The signal is strong in IB and bright in IF and IHC.
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FH SITING (Verified Customer) (08-31-2020) | excellent antibody
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