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STOML2 Polyklonaler Antikörper

STOML2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10348-1-AP

Synonyme

SLP2, SLP 2, Paratarg-7, Paraprotein target 7, EPB72-like protein 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, C6-Zellen, HEK-293-Zellen, Rattenhirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Magenkrebsgewebe, humanes Endometriumkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10348-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA STOML2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen STOML2 fusion protein Ag0363
Vollständiger Name stomatin (EPB72)-like 2
Berechnetes Molekulargewicht 356 aa, 39 kDa
Beobachtetes Molekulargewicht 39 kDa
GenBank-ZugangsnummerBC002442
Gene symbol STOML2
Gene ID (NCBI) 30968
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively.

Protokolle

Produktspezifische Protokolle
WB protocol for STOML2 antibody 10348-1-APProtokoll herunterladen
IHC protocol for STOML2 antibody 10348-1-APProtokoll herunterladen
IF protocol for STOML2 antibody 10348-1-APProtokoll herunterladen
IP protocol for STOML2 antibody 10348-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
humanWB

Cell Metab

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Authors - Ricarda Richter-Dennerlein
humanWB,IHC

Clin Cancer Res

Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma.

Authors - Zhang Liyong L
humanWB

EMBO Rep

The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L.

Authors - Timothy Wai
humanWB

Hum Mol Genet

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Authors - McCray Brett A BA
humanWB

J Pathol

Knockdown of stomatin-like protein 2 (STOML2) reduces the invasive ability of glioma cells through inhibition of the NF-κB/MMP-9 pathway.

Authors - Song Libing L

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Gaurav (Verified Customer) (02-15-2024)

Good antibody which works as per the description. Detects the endogenous proteins without any problem.

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293, Smooth Muscle Cells
STOML2 Antibody Western Blot,Immunoprecipitation validation (1:3000 dilution) in HEK293, Smooth Muscle Cells (Cat no:10348-1-AP)
FH

Hussein (Verified Customer) (03-23-2021)

I validated the antibody specificity using different siRNAs. The signal is strong in IB and bright in IF and IHC.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
  • Primary Antibody Dilution: WB 1:1000 (each dilution was used 3 times); IP 1:200; IHC 1:400
  • Cell Tissue Type: Breast cancer cell lines: MDA-MB-231, MDA-MB-361, BT-474, JIMT-1
FH

SITING (Verified Customer) (08-31-2020)

excellent antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: heart
STOML2 Antibody Western Blot validation (1:1000 dilution) in heart (Cat no:10348-1-AP)