Stomatin Polyklonaler Antikörper

Stomatin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12046-1-AP

Synonyme

STOM, Erythrocyte membrane protein band 7.2, EPB72, EPB7, BND7

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12046-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12046-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12046-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12046-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12046-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12046-1-AP

  IP result of anti-Stomatin (IP:12046-1-AP, 3ug; Detection:12046-1-AP 1:300) with HeLa cells lysate 2800ug.

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  IHC staining of human lung cancer using 12046-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12046-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 12046-1-AP

  Immunofluorescent analysis of A549 cells using 12046-1-AP (STOM antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, humanes Lebergewebe, humanes Lungengewebe, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12046-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Stomatin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Stomatin fusion protein Ag2676
• Vollständiger Name: stomatin
• Berechnetes Molekulargewicht: 288 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC010703
• Gene symbol: Stomatin
• Gene ID (NCBI): 2040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Publikationen

Species	Application	Title
human	WB	Front Med; Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome; Authors - Yashuang Yang
human	WB	Vox Sang; Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools.; Authors - Rubin O O
human	IHC	Chin J Cancer Res; Stomatin plays a suppressor role in non-small cell lung cancer metastasis.; Authors - Huaying An
human	WB,CoIP	Biophys J; Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains; Authors - Artem G Ayuyan