STMN3 Polyklonaler Antikörper

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• KD/KO Validated

STMN3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11311-1-AP

Synonyme

SCG10 like protein, SCLIP, Stathmin 3, stathmin like 3, STMN3

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Galerie der Validierungsdaten

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  WB analysis using 11311-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11311-1-AP (STMN3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11311-1-AP (STMN3 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11311-1-AP bindet in WB, IHC, IF, ELISA STMN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STMN3 fusion protein Ag1853
• Vollständiger Name: stathmin-like 3
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC009381
• Gene symbol: STMN3
• Gene ID (NCBI): 50861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stathmin family phosphoproteins participate in the control of microtubule dynamics and have been proposed to be involved in the control of neuronal differentiation. Neuron specific Stathmin-3 (STMN3) is a novel STAT3 (signal transducer and activator of transcription 3) interacting protein. STAT3 is a key contributor to cancer cell migration and invasion. STMN3 exhibits microtubule-destabilizing activity, which is antagonized by STAT3. SCG10 is a widely studied and recognized neuronal differentiation marker, STMN3 is a SCG10-like protein (SCLIP) and is present from the earliest stages of hippocampal neuron differentiation in culture at vesicle-like structures following dynamic microtubules. RNAi mediated inhibition of this gene resulted in increased axonal branching.

Publikationen

Species	Application	Title
human	WB, IHC	Cancer Res; Coordinated expression of stathmin family members by far upstream sequence element-binding protein-1 increases motility in non-small cell lung cancer.; Authors - Stephan Singer
human	WB	Cancer Res; RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.; Authors - Nicholas Rooney
mouse	WB,IF	Elife; HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration.; Authors - Alicia Tapias
human	WB	Mol Cancer Ther; PMID: 23543364; Authors - Xie Xuemei X ; KD Validated
mouse	WB	J Biol Chem; The Stathmin-2 membrane targeting domain is required for axon protection and regulated degradation by DLK signaling; Authors - Emma J C Thornburg-Suresh
	WB	BMC Cell Biol; CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited.; Authors - Matthew A Helsby