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STAT5A-Specific Polyklonaler Antikörper

STAT5A-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51074-2-AP

Synonyme

MGF, STAT5, STAT5A, STAT5A-Specific

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of NIH/3T3 cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of NIH/3T3 using 51074-2-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of HeLa using 51074-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of HepG2 using 51074-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human cervical cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human cervical cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human breast cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51074-2-AP (STAT5A-specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human breast cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51074-2-AP (STAT5A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human lung cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51074-2-AP (STAT5A-specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human lung cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51074-2-AP (STAT5A Antibody) at dilution of 1:50 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBNIH/3T3-Zellen, HeLa-Zellen, HepG2-Zellen
Erfolgreiche Detektion in IHChumanes Zervixkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2400
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

51074-2-AP bindet in WB, IHC, ELISA STAT5A-Specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name signal transducer and activator of transcription 5A
Berechnetes Molekulargewicht 794 aa, 92 kDa
Beobachtetes Molekulargewicht 92 kDa
GenBank-ZugangsnummerBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GH. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is specific to STAT5A. It has no cross reaction to STAT5B.

Protokolle

Produktspezifische Protokolle
WB protocol for STAT5A-Specific antibody 51074-2-APProtokoll herunterladen
IHC protocol for STAT5A-Specific antibody 51074-2-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen