STAT3 Polyklonaler Antikörper

STAT3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 51076-2-AP

Synonyme

Acute phase response factor, APRF, HIES, STAT3

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 51076-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 51076-2-AP (STAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51076-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51076-2-AP (STAT3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51076-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51076-2-AP (STAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 51076-2-AP

  IP Result of anti-STAT3 (IP:51076-2-AP, 4ug; Detection:51076-2-AP 1:500) with HeLa cells lysate 1500ug.

• 

  IHC staining of human cervical cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51076-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51076-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51076-2-AP (STAT3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51076-2-AP (STAT3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

51076-2-AP bindet in WB, IP, IHC, ELISA STAT3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Berechnetes Molekulargewicht: 770 aa, 88 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC000627
• Gene symbol: STAT3
• Gene ID (NCBI): 6774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Publikationen

Species	Application	Title
mouse	WB	FASEB J; Lmo4-resistin signaling contributes to adipose tissue-liver crosstalk upon weight cycling.; Authors - Yu Sun
mouse	WB	Neurotox Res; Hispidulin Inhibits Neuroinflammation in Lipopolysaccharide-Activated BV2 Microglia and Attenuates the Activation of Akt, NF-κB, and STAT3 Pathway.; Authors - Chung-I Yu
human	WB	Oncol Lett; ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway.; Authors - Shengjin Yu
human	IP	Cell Biol Int; Identification and analysis of glycogen synthase kinase 3 beta1 interactome.; Authors - Gao Xuejuan X
		Mol Med Rep; A small GTPase?like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in?vitro via interaction with Rac1 and Stat3.; Authors - Hu Xiaopeng X