STAT2 Monoklonaler Antikörper

STAT2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1G12C4

Kat-Nr. : 66485-1-Ig

Synonyme

ISGF 3, P113, STAT113, STAT2

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Galerie der Validierungsdaten

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  WB analysis using 66485-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66485-1-Ig (STAT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66485-1-Ig (STAT2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Ramos using 66485-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using STAT2 antibody (66485-1-Ig, Clone: 1G12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Ramos using 66485-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using STAT2 antibody (66485-1-Ig, Clone: 1G12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, COLO 320-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

66485-1-Ig bindet in WB, IP, IHC, IF, ELISA STAT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STAT2 fusion protein Ag10168
• Vollständiger Name: signal transducer and activator of transcription 2, 113kDa
• Berechnetes Molekulargewicht: 851 aa, 98 kDa
• Beobachtetes Molekulargewicht: 113 kDa
• GenBank-Zugangsnummer: BC051284
• Gene symbol: STAT2
• Gene ID (NCBI): 6773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Int Immunopharmacol; Silencing SIRT1 promotes the anti-HBV action of IFN-α by regulating Pol expression and activating the JAK-STAT signaling pathway; Authors - Qinyan Tang
mouse	WB	Exp Eye Res; Integrated analysis of murine cornea identifies JAK/STAT signaling pathway upregulated specifically in female Vitamin A Deficient mice; Authors - Hanrui Yu
pig	WB,IP	PLoS Pathog; Free ISG15 inhibits Pseudorabies virus infection by positively regulating type I IFN signaling; Authors - Huimin Liu
mouse	WB	J Nanobiotechnology; Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy; Authors - Boping Jing