STAT2 Polyklonaler Antikörper

STAT2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 51075-2-AP

Synonyme

ISGF 3, P113, STAT113, STAT2

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Galerie der Validierungsdaten

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  WB analysis of multi-cells using 51075-2-AP

  WB result of anti-STAT2 (51075-2-AP) in different cell lysates.

• 

  WB analysis of MCF-7 using 51075-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 51075-2-AP (STAT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, Multizellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

51075-2-AP bindet in WB, IHC, ELISA STAT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 2, 113kDa
• Berechnetes Molekulargewicht: 851 aa, 98 kDa
• Beobachtetes Molekulargewicht: 100-113 kDa
• GenBank-Zugangsnummer: BC051284
• Gene symbol: STAT2
• Gene ID (NCBI): 6773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human STAT2. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Publikationen

Species	Application	Title
human		J Clin Invest; Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy.; Authors - Yan-Ping Xu
human	WB	BMC Vet Res; Curcumol inhibits EMCV replication by activating CH25H and inhibiting the formation of ROs; Authors - Jiangang Zheng
human	WB	J Transl Med; Pretreatment of UC-MSCs with IFN-α2 improves treatment of liver fibrosis by recruiting neutrophils; Authors - Ye Xie
mouse	WB	Viruses; Interferon-Stimulated Gene 15 Knockout in Mice Impairs IFNα-Mediated Antiviral Activity; Authors - Chen Li