STAT2 Polyklonaler Antikörper

STAT2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 16674-1-AP

Synonyme

ISGF 3, P113, STAT113, STAT2

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Galerie der Validierungsdaten

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  WB analysis using 16674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16674-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 16674-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16674-1-AP

  IP Result of anti-STAT2 (IP:16674-1-AP, 4ug; Detection:16674-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 16674-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 16674-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 16674-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 16674-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HT-1080.Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

16674-1-AP bindet in WB, IP, IHC, IF, ELISA STAT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STAT2 fusion protein Ag10168
• Vollständiger Name: signal transducer and activator of transcription 2, 113kDa
• Berechnetes Molekulargewicht: 851 aa, 98 kDa
• Beobachtetes Molekulargewicht: 113 kDa
• GenBank-Zugangsnummer: BC051284
• Gene symbol: STAT2
• Gene ID (NCBI): 6773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT2. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; A 2-Benzylmalonate Derivative as STAT3 Inhibitor Suppresses Tumor Growth in Hepatocellular Carcinoma by Upregulating β-TrCP E3 Ubiquitin Ligase.; Authors - Ting Peng
human	WB	Oncotarget; Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.; Authors - Ighodaro Igbe
pig	WB	Int J Biol Macromol; Atractylodis macrocephalae polysaccharides protect against DSS-induced intestinal injury through a novel lncRNA ITSN1-OT1.; Authors - Xin Zong
human	WB, IP, IF	J Immunol; Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.; Authors - Mudan Zhang
human	WB	Toxicol Appl Pharmacol; PMID: 29031523; Authors - Orawan Wonganan
human	WB	Mol Cancer Res; STAT1 and NF-κB Inhibitors Diminish Basal Interferon-Stimulated Gene Expression and Improve the Productive Infection of Oncolytic HSV in MPNST Cells.; Authors - Joshua D Jackson