STAG1 Polyklonaler Antikörper

STAG1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14015-1-AP

Synonyme

Cohesin subunit SA 1, SA1, SCC3 homolog 1, STAG1, stromal antigen 1

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Galerie der Validierungsdaten

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  WB analysis of human heart using 14015-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14015-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14015-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14015-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, humanes Herzgewebe, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14015-1-AP bindet in WB, ELISA STAG1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STAG1 fusion protein Ag5123
• Vollständiger Name: stromal antigen 1
• Berechnetes Molekulargewicht: 144 kDa
• Beobachtetes Molekulargewicht: 155 kDa
• GenBank-Zugangsnummer: BC064699
• Gene symbol: STAG1
• Gene ID (NCBI): 10274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.

Publikationen

Species	Application	Title
rat	WB	Bone Res; Circular RNA circStag1 promotes bone regeneration by interacting with HuR.; Authors - Gaoyang Chen
human	WB	Proc Natl Acad Sci U S A; Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1.; Authors - Ena Oreskovic