ST7 Polyklonaler Antikörper

ST7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11945-1-AP

Synonyme

ETS7q, FAM4A, FAM4A1, HELG, Protein FAM4A1, Protein HELG, RAY1, SEN4, ST7, TSG7

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Galerie der Validierungsdaten

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  WB analysis using 11945-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11945-1-AP (ST7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11945-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11945-1-AP (ST7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11945-1-AP (ST7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11945-1-AP (ST7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 11945-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11945-1-AP (ST7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 11945-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11945-1-AP (ST7 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11945-1-AP bindet in WB, IHC, ELISA ST7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST7 fusion protein Ag2547
• Vollständiger Name: suppression of tumorigenicity 7
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC030954
• Gene symbol: ST7
• Gene ID (NCBI): 7982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human ST7 (suppressor of tumorigenicity 7) gene, also called FAM4A1, HELG or RAY1, maps to a region on chromosome 7 identified as an autism-susceptibility locus. ST7 has been proposed to be a tumor suppressor gene with highest expression in heart, liver and pancreas. The product of this gene is a multi-pass membrane protein belonging to the ST7 family, full-length ST7 cDNA has been cloned and verified in cancer cell lines, recent result suggests that ST7 may mediate tumor suppression through the regulation of the genes involved in maintaining the cellular structure of the cell and involved in oncogenic pathways.

Publikationen

Species	Application	Title
human	WB	Cancer Res; Genetic validation of the protein arginine methyltransferase PRMT5 as a candidate therapeutic target in glioblastoma.; Authors - Fengting Yan