ST3GAL4 Polyklonaler Antikörper

ST3GAL4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13546-1-AP

Synonyme

Alpha 2,3 sialyltransferase IV, Alpha 2,3 ST 4, CGS23, Gal NAc6S, NANTA3, SAT 3, SAT3, Sialyltransferase 4C, SIAT4, SIAT4 C, SIAT4C, ST 4, ST3Gal IV, ST3GAL4, ST3GalA.2, ST3GalIV, STZ

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Galerie der Validierungsdaten

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  WB analysis using 13546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13546-1-AP (ST3GAL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13546-1-AP (ST3GAL4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13546-1-AP (ST3GAL4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13546-1-AP (ST3GAL4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13546-1-AP (ST3GAL4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

13546-1-AP bindet in WB, IF, IHC, ELISA ST3GAL4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST3GAL4 fusion protein Ag3665
• Vollständiger Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 4
• Berechnetes Molekulargewicht: 333 aa, 38 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC010645
• Gene symbol: ST3GAL4
• Gene ID (NCBI): 6484
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST3GAL4, also known as CGS23, NANTA3 and SIAT4C, is a member of the glycosyltransferase 29 family. ST3GAL4 protein exists in multiple splice variants and is frequently modified by glycosylation. ST3GAL4 is expressed uniformly in tissues and organs during all stages of development, and the subcellular localization is currently not clear.

Publikationen

Species	Application	Title
human	WB, IHC	Front Oncol; ST3Gal IV Mediates the Growth and Proliferation of Cervical Cancer Cells In Vitro and In Vivo Via the Notch/p21/CDKs Pathway.; Authors - Yinshuang Wu
human	WB,IHC	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu
human	WB,IHC	FEBS Lett; The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.; Authors - Ming Yu
human	WB	Glycobiology; Sialylation-dependent interaction between PD-L1 and CD169 promotes monocytes adhesion to endothelial cells; Authors - Kebo Cai
mouse	IF	Elife; RANK+TLR2+ myeloid subpopulation converts autoimmune to joint destruction in rheumatoid arthritis; Authors - Weixin Zhang