SRPX2 Polyklonaler Antikörper

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• KD/KO Validated

SRPX2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11845-1-AP

Synonyme

Sushi repeat-containing protein SRPX2, SRPUL, RESDX, PMGX, CBPS

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 11845-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11845-1-AP (SRPX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11845-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11845-1-AP (SRPX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11845-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11845-1-AP (SRPX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11845-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11845-1-AP (SRPX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of rat brain using 11845-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SRPX2 antibody (11845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 11845-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SRPX2 antibody (11845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, HepG2-Zellen, humanes Hirngewebe, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

11845-1-AP bindet in WB, IHC, IF-P, ELISA SRPX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRPX2 fusion protein Ag2516
• Vollständiger Name: sushi-repeat-containing protein, X-linked 2
• Berechnetes Molekulargewicht: 465 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC020733
• Gene symbol: SRPX2
• Gene ID (NCBI): 27286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRPX2 (sushi-repeat-containing protein, X-linked 2) is a secreted protein expressed in neurons of the human adult brain, including the rolandic area (PMID: 20874700). Firstly described as sushi-repeat protein up-regulated in leukemia (SPRUL) in leukemia cells with dysregulated expression at the transcriptional level, SRPX2 has been recently shown as a multifunctional protein. SRPX2 is a ligand for uPAR, the urokinase-type plasminogen activator (uPA) receptor (PMID: 18718938). It is involved in seizure disorders, angiogenesis and cellular adhesion (PMID: 22242148; 19667118; 16497722). The involvement of SRPX2 in disorders of language cortex and cognition suggests it has an important role in the perisylvian region critical for language development (PMID: 16497722).

Publikationen

Species	Application	Title
human	WB	Theranostics; Local administration of liposomal-based Srpx2 gene therapy reverses pulmonary fibrosis by blockading fibroblast-to-myofibroblast transition.; Authors - Qi Wang ; KD Validated
human	WB	Hum Mol Genet; Epileptic and developmental disorders of the speech cortex: ligand/receptor interaction of wild-type and mutant SRPX2 with the plasminogen activator receptor uPAR.; Authors - Royer-Zemmour Barbara B
mouse	WB	FASEB J; Sushi repeat protein X-linked 2, a novel mediator of angiogenesis.; Authors - Miljkovic-Licina Marijana M
human	IHC	Front Cell Dev Biol; The Landscape of the Tumor Microenvironment in Skin Cutaneous Melanoma Reveals a Prognostic and Immunotherapeutically Relevant Gene Signature.; Authors - Sitong Zhou
mouse	WB	Int J Mol Sci; The Role of Lysosomes in a Broad Disease-Modifying Approach Evaluated across Transgenic Mouse Models of Alzheimer's Disease and Parkinson's Disease and Models of Mild Cognitive Impairment.; Authors - Jeannie Hwang
human	WB, IHC	Int J Clin Exp Pathol; SRPX2 promotes cell migration and invasion via FAK dependent pathway in pancreatic cancer.; Authors - Zhenyuan Gao