SRPK2 Polyklonaler Antikörper

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• KD/KO Validated

SRPK2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 25417-1-AP

Synonyme

SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2, SRPK2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 25417-1-AP

  WB result of SRPK2 antibody (25417-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRPK2 transfected HeLa cells.

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  WB analysis of HeLa using 25417-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25417-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25417-1-AP

  IP Result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 25417-1-AP

  IP Result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of mouse testis using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

25417-1-AP bindet in WB, IP, IHC, IF, ELISA SRPK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRPK2 fusion protein Ag17885
• Vollständiger Name: SFRS protein kinase 2
• Berechnetes Molekulargewicht: 688 aa, 78 kDa
• Beobachtetes Molekulargewicht: 105-110 kDa
• GenBank-Zugangsnummer: BC035214
• Gene symbol: SRPK2
• Gene ID (NCBI): 6733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).

Publikationen

Species	Application	Title
human	WB,IF	Cancer Discov; Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.; Authors - Guillaume Fonteneau