SRPK1 Polyklonaler Antikörper

SRPK1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14073-1-AP

Synonyme

SFRS protein kinase 1, SFRSK1, SR protein specific kinase 1, SRPK1

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Galerie der Validierungsdaten

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  WB analysis using 14073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14073-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14073-1-AP

  IP result of anti-SRPK1(IP:14073-1-AP, 4ug; Detection:14073-1-AP 1:500) with mouse testis tissue lysate 2040 ug.

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 14073-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 14073-1-AP (SRPK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 14073-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug SRPK1 antibody (14073-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, L02-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14073-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA SRPK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRPK1 fusion protein Ag5149
• Vollständiger Name: SFRS protein kinase 1
• Berechnetes Molekulargewicht: 92.4 kDa
• Beobachtetes Molekulargewicht: 72-95 kDa
• GenBank-Zugangsnummer: BC038292
• Gene symbol: SRPK1
• Gene ID (NCBI): 6732
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRPK1 (serine-arginine protein kinase 1) is a protein kinase that specifically phosphorylates proteins containing serine-arginine-rich domains. Its substrates include a family of SR proteins that are key regulators of mRNA AS (alternative splicing). SRPK1 may be localized both in the cytoplasm and nucleus and translocates between these compartments under various conditions (e.g. cell cycle and stress). It is able to phosphorylate SR proteins both in cytoplasm and nucleus (the significance of which remains incompletely understood) and thus regulate the activity of these proteins by affecting their ability to bind other proteins and/or RNA as well as their intracellular localization.(PMID:22817743). This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
	WB,CoIP	Cell Metab; DRAK2 aggravates nonalcoholic fatty liver disease progression through SRSF6-associated RNA alternative splicing.; Authors - Yufeng Li
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	J Cancer; Clinical and functional significance of CHK1-S, an alternatively spliced isoform of the CHK1 gene, in hepatocellular carcinoma.; Authors - Guanghui Hu
mouse	IF	BMC Cancer; MiR-659-3p inhibits osteosarcoma progression and metastasis by inhibiting cell proliferation and invasion via targeting SRPK1; Authors - Yubao Gong