SRP68 Polyklonaler Antikörper

SRP68 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11585-1-AP

Synonyme

SRP 68, Signal recognition particle subunit SRP68

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 11585-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11585-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 11585-1-AP

  IP result of anti-SRP68 (IP:11585-1-AP, 4ug; Detection:11585-1-AP 1:400) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human gliomas using 11585-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11585-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11585-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11585-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11585-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SRP68 antibody (11585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

11585-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SRP68 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRP68 fusion protein Ag2177
• Vollständiger Name: signal recognition particle 68kDa
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC020238
• Gene symbol: SRP68
• Gene ID (NCBI): 6730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of secretory proteins to the rough endoplasmic reticulum (ER) membrane. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. SRP68 is one component of the ribonucleprotein complex, and forms a heterodimer with SRP72.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis.; Authors - Mehdi Pirouz
	WB	Nucleic Acids Res; The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins.; Authors - Joori Park
human	WB	Nucleic Acids Res; Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.; Authors - Piazzon Nathalie N
human	WB	Nucleic Acids Res; Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction.; Authors - Benjamin Rothé
human	WB	PLoS One; Decreased dicer expression enhances SRP-mediated protein targeting.; Authors - Ren Yong-Feng YF
human	WB	PLoS One; The CELF1 RNA-Binding Protein Regulates Decay of Signal Recognition Particle mRNAs and Limits Secretion in Mouse Myoblasts.; Authors - Joseph Russo