SRP54 Polyklonaler Antikörper

SRP54 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11729-1-AP

Synonyme

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4

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Galerie der Validierungsdaten

• 

  WB analysis of human placenta using 11729-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11729-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11729-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 11729-1-AP

  IP result of anti-SRP54 (IP:11729-1-AP, 4ug; Detection:11729-1-AP 1:300) with human placenta tissue lysate 1015 ug.

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11729-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (11729-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, NIH/3T3-Zellen
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

11729-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SRP54 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRP54 fusion protein Ag2327
• Vollständiger Name: signal recognition particle 54kDa
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC003389
• Gene symbol: SRP54
• Gene ID (NCBI): 6729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Publikationen

Species	Application	Title
human		Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	PLoS One; Decreased dicer expression enhances SRP-mediated protein targeting.; Authors - Ren Yong-Feng YF
human	WB	Biochem Biophys Res Commun; Polyglutamine expansion disturbs the endoplasmic reticulum formation, leading to caspase-7 activation through Bax.; Authors - Masashi Ueda
human	WB	Biochem Biophys Res Commun; Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion.; Authors - Masashi Ueda
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi