SRGAP1 Polyklonaler Antikörper

SRGAP1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13252-1-AP

Synonyme

ARHGAP13, KIAA1304, SRGAP1

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Galerie der Validierungsdaten

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  WB analysis using 13252-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13252-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 13252-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13252-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13252-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13252-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 13252-1-AP

  IP result of anti-SRGAP1 (IP:13252-1-AP, 4ug; Detection:13252-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Apoptotisierte HeLa-Zellen, HEK-293-Zellen, Maushirngewebe, Mausnierengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

13252-1-AP bindet in WB, IP, IF, IHC, ELISA SRGAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRGAP1 fusion protein Ag4104
• Vollständiger Name: SLIT-ROBO Rho GTPase activating protein 1
• Berechnetes Molekulargewicht: 1085 aa, 124 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC029919
• Gene symbol: SRGAP1
• Gene ID (NCBI): 57522
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF,IP	J Am Soc Nephrol; SRGAP1 Controls Small Rho GTPases To Regulate Podocyte Foot Process Maintenance.; Authors - Manuel Rogg
human	WB	J Exp Clin Cancer Res; srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer.; Authors - Yuyang Feng
human	WB,IF	Cell Rep; A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling; Authors - Chandrani Mondal