FDFT1 Polyklonaler Antikörper

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• KD/KO Validated

FDFT1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 13128-1-AP

Synonyme

SQS, Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21

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Galerie der Validierungsdaten

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  WB analysis using 13128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13128-1-AP (FDFT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13128-1-AP

  WB result of FDFT1 antibody (13128-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDFT1 transfected HepG2 cells.

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  IP experiment of COLO 320 using 13128-1-AP

  IP result of anti-FDFT1 (IP:13128-1-AP, 4ug; Detection:13128-1-AP 1:500) with COLO 320 cells lysate 2400ug.

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  IF Staining of L02 using 13128-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 13128-1-AP (FDFT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, T-47D-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 14 publications below
• IHC: See 3 publications below

Produktinformation

13128-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA FDFT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FDFT1 fusion protein Ag3743
• Vollständiger Name: farnesyl-diphosphate farnesyltransferase 1
• Berechnetes Molekulargewicht: 417 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC029641
• Gene symbol: FDFT1
• Gene ID (NCBI): 2222
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Farnesyl-diphosphate farnesyltransferase 1 (FDFT1), also known as SQS, is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).

Publikationen

Species	Application	Title
human,mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	Cell Metab; Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer.; Authors - Douglas E Biancur ; KD Validated
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	WB	Cancer Res; Caffeine supplementation and FOXM1 inhibition enhance the antitumor effect of statins in neuroblastoma; Authors - Gia-Buu Tran ; KD Validated
human	WB	Phytomedicine; Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.; Authors - Xing Yue Xu
human	WB	J Inherit Metab Dis; Identification of FDA-approved drugs that increase mevalonate kinase in hyper IgD syndrome; Authors - Frouwkje A Politiek