SQLE Polyklonaler Antikörper

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• KD/KO Validated

SQLE Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

80

Kat-Nr. : 12544-1-AP

Synonyme

Squalene monooxygenase, squalene epoxidase, ERG1, EC:1.14.14.17

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Galerie der Validierungsdaten

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  WB analysis using 12544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12544-1-AP (SQLE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12544-1-AP

  IP result of anti-SQLE (IP:12544-1-AP, 4ug; Detection:12544-1-AP 1:500) with HepG2 cells lysate 2240 ug.

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  IHC staining of human prostate cancer using 12544-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12544-1-AP (SQLE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12544-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12544-1-AP (SQLE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12544-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SQLE antibody (12544-1-AP) at dilution of 1:40 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of PC-3 using 12544-1-AP

  Immunofluorescent analysis of PC-3 cells using 12544-1-AP (SQLE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 72 publications below
• IHC: See 15 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

12544-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA SQLE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SQLE fusion protein Ag3266
• Vollständiger Name: squalene epoxidase
• Berechnetes Molekulargewicht: 574 aa, 64 kDa
• Beobachtetes Molekulargewicht: 50-64 kDa
• GenBank-Zugangsnummer: BC017033
• Gene symbol: SQLE
• Gene ID (NCBI): 6713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
hamster	WB	Cell Metab; Cholesterol-dependent degradation of squalene monooxygenase, a control point in cholesterol synthesis beyond HMG-CoA reductase.; Authors - Gill Saloni S
mouse	WB	Cell Metab; PMID: 21109190; Authors - Ryo Suzuki
mouse	WB	PLoS Biol; Reduction of the cholesterol sensor SCAP in the brains of mice causes impaired synaptic transmission and altered cognitive function.; Authors - Suzuki Ryo R
human	WB,IHC	Nat Commun; MiR-205-driven downregulation of cholesterol biosynthesis through SQLE-inhibition identifies therapeutic vulnerability in aggressive prostate cancer.; Authors - C Kalogirou
human	WB	Redox Biol; Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation; Authors - Youzi Kong

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