SPRY4 Polyklonaler Antikörper

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• KD/KO Validated

SPRY4 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 22765-1-AP

Synonyme

Protein sprouty homolog 4, sprouty homolog 4 (Drosophila), Spry 4, SPRY4

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Galerie der Validierungsdaten

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  WB analysis of A375 using 22765-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22765-1-AP (SPRY4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 22765-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22765-1-AP (SPRY4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 22765-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22765-1-AP (SPRY4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 22765-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22765-1-AP (SPRY4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 22765-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22765-1-AP (SPRY4 Antibody) at dilution of 1:50 (under 10x lens).

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  FC experiment of HeLa using 22765-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SPRY4 antibody (22765-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

22765-1-AP bindet in WB, IHC, IF, FC, ELISA SPRY4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPRY4 fusion protein Ag18574
• Vollständiger Name: sprouty homolog 4 (Drosophila)
• Berechnetes Molekulargewicht: 299 aa, 33 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC125095
• Gene symbol: SPRY4
• Gene ID (NCBI): 81848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPRY4, also named as Protein sprouty homolog 4, is a 299 amino acid protein, which contains one SPR (sprouty) domain and belongs to the sprouty family. SPRY4 Suppresses the INS receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. SPRY4 has a cellular function to regulate the actin cytoskeleton, independent of its inhibitory activity on the Ras/MAP kinase signalling. SPRY1, SPRY2, SPRY3, SPRY4, are central and complex regulators of the receptor tyrosine kinase (RTK) signalling pathway.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Targeting enhancer reprogramming to mitigate MEK inhibitor resistance in preclinical models of advanced ovarian cancer.; Authors - Shini Liu ; KD Validated
human	IHC	Cancer Lett; Coupling HDAC4 with transcriptional factor MEF2D abrogates SPRY4-mediated suppression of ERK activation and elicits hepatocellular carcinoma drug resistance.; Authors - Qingxia Ma ; KO Validated
human	WB,IHC,IF	Am J Reprod Immunol; SPRY4 regulates trophoblast proliferation and apoptosis via regulating IFN-γ-induced STAT1 expression and activation in recurrent miscarriage.; Authors - Shi Qin ; KD Validated
human	WB,IHC	Cell Death Dis; SPRY4 is responsible for pathogenesis of adolescent idiopathic scoliosis by contributing to osteogenic differentiation and melatonin response of bone marrow-derived mesenchymal stem cells.; Authors - Jing Li ; KD Validated
human	WB	Nat Commun; AXL confers intrinsic resistance to osimertinib and advances the emergence of tolerant cells.; Authors - Hirokazu Taniguchi ; KD Validated
human	WB	Adipocyte; SPRY4 promotes adipogenic differentiation of human mesenchymal stem cells through the MEK-ERK1/2 signaling pathway; Authors - Na Li ; KD Validated