SPRY2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPRY2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (2)

Kat-Nr. : 11383-1-AP

Synonyme

Spry-2, Spry 2, sprouty homolog 2 (Drosophila), Protein sprouty homolog 2, hSPRY2

---

Galerie der Validierungsdaten

• 

  WB analysis using 11383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11383-1-AP (SPRY2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 11383-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 11383-1-AP (SPRY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11383-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11383-1-AP (SPRY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11383-1-AP (SPRY2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11383-1-AP (SPRY2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A375 using 11383-1-AP

  Immunofluorescent analysis of A375 cells using 11383-1-AP (SPRY2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SW 1990-Zellen, A375-Zellen, Jurkat-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11383-1-AP bindet in WB, IHC, IF/ICC, ELISA SPRY2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPRY2 fusion protein Ag1918
• Vollständiger Name: sprouty homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa, 60~65 kDa
• GenBank-Zugangsnummer: BC015745
• Gene symbol: SPRY2
• Gene ID (NCBI): 10253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sprouty(SPRY) genes encode intracellular inhibitors of receptor tyrosine kinase (RTK) signaling pathways, including those triggered by fibroblast growth factors (Fgfs). In addition to its well-reported function as an inhibitor of the RAS/RAF/MEK signalling cascade, SPRY2 exerts its inhibitory role in the PI3K/AKT signalling pathway through PTEN, and inhibits ERK/MAPK activity via EGFR trafficking. SPRY2 was detected 60-65 kDa (PMID:29291435).

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.; Authors - Guangying Yang
human	WB	J Cell Mol Med; Exosomal miR-27 negatively regulates ROS production and promotes granulosa cells apoptosis by targeting SPRY2 in OHSS.; Authors - Kailu Liu ; KD Validated
human	WB	Toxicol Appl Pharmacol; Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.; Authors - Nai-Jie Fu
mouse	WB	Cell Biol Int; Sprouty2 is involved in the control of osteoblast proliferation and differentiation through the FGF and BMP signaling pathways.; Authors - Takaharu Taketomi
human,mouse	WB,IHC,IF	Breast Cancer Res; Loss of SPRY2 contributes to cancer-associated fibroblasts activation and promotes breast cancer development; Authors - Huijuan Dai ; KD Validated
human	WB	Cancer Cell Int; Therapeutic effect and transcriptome-methylome characteristics of METTL3 inhibition in liver hepatocellular carcinoma; Authors - Qingbin Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Christine (Verified Customer) (01-27-2023)

Detects two bands on HEK293T lysates. main one between 36 and 48 kDa, second one between 48 and 85 kDa, in accordance with datasheet

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293T

FH

Silvia (Verified Customer) (01-31-2022)

The antibody worked really well and gave me a clear band on Western Blot around 50 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Human adherent cancer cells