Spartin, SPG20 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Spartin, SPG20 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 13791-1-AP

Synonyme

KIAA0610, SPARTIN, Spartin, SPG20, Spastic paraplegia 20 protein, SPG20, TAHCCP1

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Galerie der Validierungsdaten

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  WB analysis using 13791-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13791-1-AP

  WB result of Spartin, SPG20 antibody (13791-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Spartin, SPG20 transfected HeLa cells.

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  IP experiment of HeLa using 13791-1-AP

  IP result of anti-Spartin, SPG20(IP:13791-1-AP, 4ug; Detection:13791-1-AP 1:2000) with HeLa cells lysate 2320 ug.

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  IHC staining of human liver cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 13791-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Spartin, SPG20 (13791-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

13791-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Spartin, SPG20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Spartin, SPG20 fusion protein Ag4815
• Vollständiger Name: spastic paraplegia 20 (Troyer syndrome)
• Berechnetes Molekulargewicht: 75 kDa, 85 kDa
• Beobachtetes Molekulargewicht: 75-84 kDa
• GenBank-Zugangsnummer: BC047083
• Gene symbol: SPG20
• Gene ID (NCBI): 23111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.

Publikationen

Species	Application	Title
human	WB	Ann Neurol; Developmental and degenerative features in a complicated spastic paraplegia.; Authors - Manzini M Chiara MC
human	WB	Elife; Chromosome mis-segregation and cytokinesis failure in trisomic human cells.; Authors - Joshua M Nicholson
mouse	WB,IF	Hum Mol Genet; Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.; Authors - Renvoisé Beno?t B ; KO Validated
human	WB,IF	Oncogene; SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.; Authors - Lind G E GE
mouse	WB	J Cell Sci; Germline Deletion of Huntingtin Causes Male Infertility and Arrested Spermiogenesis in Mice.; Authors - Jinting Yan
human	WB,IF	FASEB J; A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism.; Authors - Chiara Diquigiovanni ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

beyenech (Verified Customer) (11-22-2019)

The antibody works also for Immunoprecipitation

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: IF: 1:100; WB:1:1000
• Cell Tissue Type: 293T and HeLa

Spartin, SPG20 Antibody Western Blot,Immunofluorescence, validation (IF: 1:100; WB:1:1000 dilution) in 293T and HeLa (Cat no:13791-1-AP)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C