SOX6 Polyklonaler Antikörper

SOX6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14010-1-AP

Synonyme

HSSOX6, SOX6, SOXD, Transcription factor SOX 6

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Galerie der Validierungsdaten

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  WB analysis using 14010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14010-1-AP (SOX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14010-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14010-1-AP (SOX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, C6-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 3 publications below

Produktinformation

14010-1-AP bindet in WB, IF, IHC, ELISA SOX6 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOX6 fusion protein Ag5111
• Vollständiger Name: SRY (sex determining region Y)-box 6
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC047064
• Gene symbol: SOX6
• Gene ID (NCBI): 55553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Sex-determining Region Y (SRY)-related high-mobilitygroup box 6 (SOX6) is a transcription factor that was discovered in 1990s while searching for Sry-related genes. SOX6 belongs to the subfamily D of SOX protein and can recognize the conserved sequence of "(A/T)(A/T)CAA(A/ T)G" within the transcriptional regulation region of the target gene with its HMG domain. Sox6 contains DNA binding domain and binds to the minor groove of DNA. The ability of SOX6 to interact with different proteins at different places and times, makes it an intricate protein with roles in multiple processes. SOX6 has been implicated in diabetic nephropathy, adipogenesis, cardiomyopathy, inflammatory bowel disease, and cancer. (PMID: 33230925)

Publikationen

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	WB	Nat Commun; VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.; Authors - Yuki Miyamoto
mouse	WB	Int J Mol Med; miRNA‑342 suppresses renal interstitial fibrosis in diabetic nephropathy by targeting SOX6.; Authors - Zhen-Huan Jiang
human	WB	Nephrology (Carlton); CircHOMER1 aggravates oxidative stress, inflammation and ECM deposition in high glucose-induced human mesangial cells; Authors - Shi Shu
	IF	bioRxiv; Eed controls craniofacial osteoblast differentiation and mesenchymal proliferation from the neural crest; Authors - Tim Casey-Clyde
human	WB	Biochem Biophys Res Commun; MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway.; Authors - Wang Li