SOX10 Monoklonaler Antikörper

SOX10 Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

1D2C8

Kat-Nr. : CL594-66786

Synonyme

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM

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  IF Staining of mouse brain using CL594-66786

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594-conjugated SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:100.

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  IF Staining of mouse brain using CL594-66786

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200. DAPI (blue).

• 

  IF Staining of rat brain using CL594-66786

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200.

• 

  IF Staining of rat brain using CL594-66786

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200.

• 

  FC experiment of C6 using CL594-66786

  1X10^6 C6 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SOX10 (CL594-66786, Clone:1D2C8) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): C6-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-66786 bindet in IF-P, FC (Intra) SOX10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SOX10 fusion protein Ag23191
• Vollständiger Name: SRY (sex determining region Y)-box 10
• Berechnetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002824
• Gene symbol: SOX10
• Gene ID (NCBI): 6663
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Suppressing recurrence in Sonic Hedgehog subgroup medulloblastoma using the OLIG2 inhibitor CT-179; Authors - Yuchen Li