SOX10 Polyklonaler Antikörper

SOX10 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), IP, CoIP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 10422-1-AP

Synonyme

WS2E, Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10422-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10422-1-AP (SOX10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10422-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10422-1-AP (SOX10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10422-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10422-1-AP (SOX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse colon using 10422-1-AP

  IP result of anti-SOX10 (IP:10422-1-AP, 4ug; Detection:10422-1-AP 1:2000) with mouse colon tissue lysate 4000 ug.

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  IHC staining of human brain using 10422-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10422-1-AP (SOX10 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 10422-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10422-1-AP (SOX10 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of C6 using 10422-1-AP

  1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human SOX10 (10422-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Dünndarmgewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 14 publications below
• CoIP: See 1 publications below

Produktinformation

10422-1-AP bindet in WB, IHC, FC (Intra), IP, CoIP, ELISA, IF SOX10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOX10 fusion protein Ag0673
• Vollständiger Name: SRY (sex determining region Y)-box 10
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC002824
• Gene symbol: SOX10
• Gene ID (NCBI): 6663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Publikationen

Species	Application	Title
mouse	IHC	Nature; Regulation of intestinal immunity and tissue repair by enteric glia; Authors - Fränze Progatzky
human	IF	Front Immunol; Machine learning-based identification of SOX10 as an immune regulator of macrophage in gliomas; Authors - Gelei Xiao
mouse	WB	J Cereb Blood Flow Metab; The role of serum/glucocorticoid-regulated kinase 1 in brain function following cerebral ischemia; Authors - Celeste Yin-Chieh Wu
rat	IF	Sci Rep; Schwann cell-encapsulated chitosan-collagen hydrogel nerve conduit promotes peripheral nerve regeneration in rodent sciatic nerve defect models; Authors - Hiroaki Takeya
human	IF	J Mol Med (Berl); MESP2 variants contribute to conotruncal heart defects by inhibiting cardiac neural crest cell proliferation.; Authors - Erge Zhang
mouse	IHC	Genes Cells; TAZ inhibits acinar cell differentiation but promotes immature ductal cell proliferation in adult mouse salivary glands.; Authors - Yosuke Miyachi