SORD Monoklonaler Antikörper

SORD Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4A8E1

Kat-Nr. : 67625-1-Ig

Synonyme

L iditol 2 dehydrogenase, SDH, sorbitol dehydrogenase, SORD, SORD1

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Galerie der Validierungsdaten

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  WB analysis using 67625-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of HSC-T6 using 67625-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67625-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67625-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below

Produktinformation

67625-1-Ig bindet in WB, ELISA SORD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SORD fusion protein Ag8651
• Vollständiger Name: sorbitol dehydrogenase
• Berechnetes Molekulargewicht: 357 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC021085
• Gene symbol: SORD
• Gene ID (NCBI): 6652
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.

Publikationen

Species	Application	Title
mouse	WB	J Nutr; Sucrose intake elevates erythritol in plasma and urine in male mice; Authors - Semira R Ortiz ; KO Validated
human	WB	Front Nutr; Erythritol synthesis is elevated in response to oxidative stress and regulated by the non-oxidative pentose phosphate pathway in A549 cells; Authors - Semira R. Ortiz ; KD Validated