SORBS1 Polyklonaler Antikörper

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• KD/KO Validated

SORBS1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13854-1-AP

Synonyme

c Cbl associated protein, CAP, Ponsin, R85FL, SH3 domain protein 5, SH3D5, SH3P12, SORB1, SORBS1

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Galerie der Validierungsdaten

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  WB analysis of 3T3-L1 using 13854-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13854-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13854-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13854-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13854-1-AP

  IP result of anti-SORBS1 (IP:13854-1-AP, 4ug; Detection:13854-1-AP 1:800) with mouse liver tissue lysate 4800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 3T3-L1-Zellen, HEK-293-Zellen, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

13854-1-AP bindet in WB, IP, IF, IHC, ELISA SORBS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SORBS1 fusion protein Ag4828
• Vollständiger Name: sorbin and SH3 domain containing 1
• Berechnetes Molekulargewicht: 1292 aa, 142 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC042612
• Gene symbol: SORBS1
• Gene ID (NCBI): 10580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB，IHC	Front Cell Dev Biol; Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.; Authors - Yiyang Xu ; KD Validated
mouse	IF	Front Cell Neurosci; Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1.; Authors - Anchel González-Barriga
canine	IF	PLoS One; Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells.; Authors - Karin Fredriksson-Lidman
human,mouse	WB	Sci Rep; STAP-2 facilitates insulin signaling through binding to CAP/c-Cbl and regulates adipocyte differentiation; Authors - Yuichi Sekine
mouse	WB	PLoS One; Proteomic changes in the hippocampus of large mammals after total-body low dose radiation; Authors - Diego Iacono