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SNX9 Polyklonaler Antikörper
SNX9 Polyklonal Antikörper für IF, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15721-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe |
Erfolgreiche IP | Mausherzgewebe |
Erfolgreiche Detektion in IF | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunfluoreszenz (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 5 publications below |
WB | See 10 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Produktinformation
15721-1-AP bindet in WB, IP, IHC, IF, ELISA SNX9 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | SNX9 fusion protein Ag8440 |
Vollständiger Name | sorting nexin 9 |
Berechnetes Molekulargewicht | 595 aa, 67 kDa |
Beobachtetes Molekulargewicht | 78 kDa |
GenBank-Zugangsnummer | BC005022 |
Gene symbol | SNX9 |
Gene ID (NCBI) | 51429 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).
Protokolle
Produktspezifische Protokolle | |
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WB protocol for SNX9 antibody 15721-1-AP | Protokoll herunterladen |
IF protocol for SNX9 antibody 15721-1-AP | Protokoll herunterladen |
IP protocol for SNX9 antibody 15721-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Immunity cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity | ||
Nat Commun Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs.
| ||
Cancer Discov In vivo role of INPP4B in tumor and metastasis suppression through regulation of PI3K/AKT signaling at endosomes. | ||
Dev Cell Mitochondrial-derived vesicles compensate for loss of LC3-mediated mitophagy.
| ||
Proc Natl Acad Sci U S A Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease. | ||
Oncotarget Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice.
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