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  • KD/KO Validated

SNX9 Polyklonaler Antikörper

SNX9 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15721-1-AP

Synonyme

MST155, MSTP155, Protein SDP1, SDP1, SH3PX1, SH3PXD3A, SNX9, sorting nexin 9, WISP



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
Erfolgreiche IPMausherzgewebe
Erfolgreiche Detektion in IFHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15721-1-AP bindet in WB, IP, IHC, IF, ELISA SNX9 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SNX9 fusion protein Ag8440
Vollständiger Name sorting nexin 9
Berechnetes Molekulargewicht 595 aa, 67 kDa
Beobachtetes Molekulargewicht78 kDa
GenBank-ZugangsnummerBC005022
Gene symbol SNX9
Gene ID (NCBI) 51429
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).

Protokolle

Produktspezifische Protokolle
WB protocol for SNX9 antibody 15721-1-APProtokoll herunterladen
IF protocol for SNX9 antibody 15721-1-APProtokoll herunterladen
IP protocol for SNX9 antibody 15721-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Immunity

cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity

Authors - Yunlong Zhao
mouseWB

Nat Commun

Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs.

Authors - Kiran Todkar
  • KD Validated
humanIF

Cancer Discov

In vivo role of INPP4B in tumor and metastasis suppression through regulation of PI3K/AKT signaling at endosomes.

Authors - Chen Li Chew
humanWB

Dev Cell

Mitochondrial-derived vesicles compensate for loss of LC3-mediated mitophagy.

Authors - Christina G Towers
  • KD Validated
mouseWB

Proc Natl Acad Sci U S A

Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.

Authors - Takahiro Kawasaki
mouseWB

Oncotarget

Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice.

Authors - Chengcheng Liu
  • KO Validated