SNX25 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNX25 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, CoIP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13294-1-AP

Synonyme

MSTP043, SBBI31, SNX25, sorting nexin 25

---

Galerie der Validierungsdaten

• 

  WB analysis using 13294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13294-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13294-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EL-4 cells, C6-Zellen, Jurkat-Zellen, Maushirngewebe, Maus-Thymusgewebe, Raji-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13294-1-AP bindet in WB, IHC, CoIP, ELISA, IF SNX25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX25 fusion protein Ag3995
• Vollständiger Name: sorting nexin 25
• Berechnetes Molekulargewicht: 98 kDa
• Beobachtetes Molekulargewicht: 98-105 kDa, 65 kDa
• GenBank-Zugangsnummer: BC029868
• Gene symbol: SNX25
• Gene ID (NCBI): 83891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNXs (sorting nexin), a family of proteins playing roles in cargo sorting and signaling from compartments within the endocytic network, regulate traffic of membrane proteins including TGF-β receptors. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-β signaling through endosomal sorting of TGF-β receptors for lysosomal degradation.This antibody can recognize two isoforms: 98-105 kDa and 65 kDa.

Publikationen

Species	Application	Title
mouse	IHC	Brain Struct Funct; Neural expression of sorting nexin 25 and its regulation of tyrosine receptor kinase B trafficking.; Authors - Shoko Takemura
mouse	WB	PLoS One; SNX25 regulates proinflammatory cytokine expression via the NF-κB signal in macrophages.; Authors - Kazuya Nishimura ; KD Validated
mouse	WB	Neurosci Lett; Circadian rhythms of sorting nexin 25 in the mouse suprachiasmatic nucleus.; Authors - Shoko Takemura
mouse	WB,IF	Nat Immunol; Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway; Authors - Tatsuhide Tanaka
human	WB,CoIP	Redox Biol; Redox-modulated SNX25 as a novel regulator of GPCR-G protein signaling from endosomes; Authors - Yulong Zhang