SNX1 Polyklonaler Antikörper

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• KD/KO Validated

SNX1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10304-1-AP

Synonyme

HsT17379, SNX1, SNX1A, sorting nexin 1, Vps5

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Galerie der Validierungsdaten

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  WB analysis using 10304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10304-1-AP (SNX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10304-1-AP

  IP result of anti-SNX1 (IP:10304-1-AP, 4ug; Detection:10304-1-AP 1:800) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 10304-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10304-1-AP (SNX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10304-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10304-1-AP (SNX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 10304-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10304-1-AP (SNX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10304-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10304-1-AP (SNX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 10304-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10304-1-AP (SNX1 antibody) at dilution of 1:100 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

10304-1-AP bindet in WB, IP, IF, IHC, ELISA SNX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX1 fusion protein Ag0499
• Vollständiger Name: sorting nexin 1
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC000357
• Gene symbol: SNX1
• Gene ID (NCBI): 6642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexin 1 (SNX1, synonyms: SNX1A, MGC8664, HsT17379) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. SNX1 also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members.

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Retromer dysfunction in amyotrophic lateral sclerosis.; Authors - Eduardo J Pérez-Torres
human	WB,IP	J Neurosci; A critical role for Sorting Nexin 1 in the trafficking of metabotropic glutamate receptors.; Authors - Rohan Sharma ; KD Validated
mouse	WB	FASEB J; Sorting nexin 1 loss results in increased oxidative stress and hypertension.; Authors - Jian Yang ; KO Validated
mouse	WB	J Biol Chem; PMID: 23152498; Authors - Villar Van Anthony M VA ; KD Validated
mouse	IF	Mol Biol Cell; Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.; Authors - Gomez Timothy S TS
mouse	IF	PLoS One; PMID: 25181483; Authors - Takeshi Yamasaki