SNRPN Polyklonaler Antikörper

SNRPN Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 11070-1-AP

Synonyme

Sm-N, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D

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Galerie der Validierungsdaten

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  WB analysis using 11070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11070-1-AP (SNRPN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11070-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11070-1-AP (SNRPN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11070-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11070-1-AP (SNRPN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 11070-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using SNRPN antibody 11070-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

11070-1-AP bindet in WB, IHC, IF/ICC, ELISA SNRPN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNRPN fusion protein Ag1503
• Vollständiger Name: small nuclear ribonucleoprotein polypeptide N
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC010057
• Gene symbol: SNRPN
• Gene ID (NCBI): 6638
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome.

Publikationen

Species	Application	Title
mouse	WB	Nat Med; Targeting the histone methyltransferase G9a activates imprinted genes and improves survival of a mouse model of Prader-Willi syndrome.; Authors - Yuna Kim
human	WB	J Cell Sci; Neurochondrin interacts with the SMN protein suggesting a novel mechanism for spinal muscular atrophy pathology.; Authors - Luke W Thompson
human,mouse	WB,IF	Genes (Basel); The Diagnostic Journey of a Patient with Prader-Willi-Like Syndrome and a Unique Homozygous SNURF-SNRPN Variant; Bio-Molecular Analysis and Review of the Literature.; Authors - Karlijn Pellikaan
bovine	IF	Chin Med J (Engl); Difference of gene expression between the central and the peripheral epithelia of the bovine lens.; Authors - Ma Xuan X
human	IF	Int J Mol Sci; N-Acetylglucosamine Kinase-Small Nuclear Ribonucleoprotein Polypeptide N Interaction Promotes Axodendritic Branching in Neurons via Dynein-Mediated Microtubule Transport; Authors - Binod Timalsina

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (08-23-2022)

Antibody recognises SmN, even when expressed at low levels, but can't discriminate between SmN and its paralog SmB/B'.

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: HeLa cells transfected with Twin-Strep tagged SmN

SNRPN Antibody Western Blot validation (1:800 dilution) in HeLa cells transfected with Twin-Strep tagged SmN (Cat no:11070-1-AP)