SNRPB2 Polyklonaler Antikörper

SNRPB2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (2)

Kat-Nr. : 13512-1-AP

Synonyme

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B''

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13512-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13512-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13512-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13512-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13512-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13512-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13512-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13512-1-AP

  IP result of anti-SNRPB2 (IP:13512-1-AP, 4ug; Detection:13512-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 13512-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13512-1-AP (SNRPB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13512-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13512-1-AP (SNRPB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 13512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13512-1-AP (SNRPB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13512-1-AP

  Immunofluorescent analysis of Hela cells, using SNRPB2 antibody 13512-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Lebergewebe, humanes Nierengewebe, Mauslebergewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

13512-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SNRPB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNRPB2 fusion protein Ag4436
• Vollständiger Name: small nuclear ribonucleoprotein polypeptide B''
• Berechnetes Molekulargewicht: 225 aa, 26, 5 kDa
• Beobachtetes Molekulargewicht: 25-31 kDa
• GenBank-Zugangsnummer: BC036737
• Gene symbol: SNRPB2
• Gene ID (NCBI): 6629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

Publikationen

Species	Application	Title
human	WB	Nature; CPEB1 coordinates alternative 3'-UTR formation with translational regulation.; Authors - Felice-Alessio Bava
human	WB,IHC	Sci Transl Med; Comment on "Drug screening for ALS using patient-specific induced pluripotent stem cells".; Authors - Bilada Bilican
human	IF	Sci Transl Med; Drug screening for ALS using patient-specific induced pluripotent stem cells.; Authors - Naohiro Egawa
human	WB	Nucleic Acids Res; Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways.; Authors - Li Chen
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	STAR Protoc; Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.; Authors - Li Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Melissa (Verified Customer) (03-16-2020)

This antibody works incredibly robust in western blot analysis. However, we do observe a non-specific band in all of our blots which we have attributed to snRPA due to the size and sequence similarity of the immunogen.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa Cell Nuclear Extract

FH

John (Verified Customer) (12-06-2018)

• Applications: Western Blot,
• Cell Tissue Type: HeLa

SNRPB2 Antibody Western Blot, validation ( dilution) in HeLa (Cat no:13512-1-AP)