SNRNP200 Polyklonaler Antikörper

SNRNP200 Polyklonal Antikörper für FC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 23875-1-AP

Synonyme

ASCC3L1, BRR2, BRR2 homolog, HELIC2, KIAA0788, SNRNP200, U5 200KD

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 23875-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23875-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23875-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23875-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23875-1-AP

  IP Result of anti-SNRNP200 (IP:23875-1-AP, 4ug; Detection:23875-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  FC experiment of A431 using 23875-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human SNRNP200 (23875-1-AP) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

23875-1-AP bindet in WB, IP, FC, ELISA SNRNP200 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNRNP200 fusion protein Ag20748
• Vollständiger Name: small nuclear ribonucleoprotein 200kDa (U5)
• Berechnetes Molekulargewicht: 494 aa, 57 kDa
• Beobachtetes Molekulargewicht: 200-245 kDa
• GenBank-Zugangsnummer: BC001417
• Gene symbol: SNRNP200
• Gene ID (NCBI): 23020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
human	IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen