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SNAP25 Polyklonaler Antikörper

SNAP25 Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10007-1-AP

Synonyme

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of mouse brain tissue using SNAP25 Polyclonal antibody (10007-1-AP)

    WB analysis of mouse brain using 10007-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10007-1-AP (SNAP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SNAP25 Polyclonal antibody (10007-1-AP)

    WB analysis of mouse brain using 10007-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10007-1-AP (SNAP25 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Produktinformation

10007-1-AP bindet in ELISA SNAP25 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name synaptosomal-associated protein, 25kDa
Berechnetes Molekulargewicht 23 kDa
GenBank-ZugangsnummerBC010647
Gene symbol SNAP25
Gene ID (NCBI) 6616
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.

Protokolle

Produktspezifische Protokolle
WB protocol for SNAP25 antibody 10007-1-APProtokoll herunterladen
Standard-Protokolle
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