SMYD3 Monoklonaler Antikörper

SMYD3 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

CloneNo.

1B5C10

Kat-Nr. : 66330-1-Ig

Synonyme

SMYD3, ZMYND1, ZNFN3A1

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Galerie der Validierungsdaten

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  WB analysis of A431 using 66330-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66330-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66330-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 66330-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 66330-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SMYD3 antibody (66330-1-Ig, Clone: 1B5C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, COLO 320-Zellen, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66330-1-Ig bindet in WB, IHC, IF SMYD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMYD3 fusion protein Ag2624
• Vollständiger Name: SET and MYND domain containing 3
• Berechnetes Molekulargewicht: 428aa,49 kDa; 369aa,42 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC031010
• Gene symbol: SMYD3
• Gene ID (NCBI): 64754
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer(PMID:16155568). Mainly cytoplasmic when cells are arrested at G0/G1, accumulates in the nucleus at S phase and G2/M(Uniprot).