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SMN (Human,Mouse,Rat) Monoklonaler Antikörper

SMN (Human,Mouse,Rat) Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C10B5

Kat-Nr. : CL488-60154

Synonyme

C BCD541, Component of gems 1, FLJ76644, Gemin 1, SMN, SMN (Human,Mouse,Rat), SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SMN (Human,Mouse,Rat (CL488-60154)

    IF Staining of HepG2 using CL488-60154

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SMN (Human,Mouse,Rat) antibody (CL488-60154, Clone: 3C10B5 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated SMN (Human,Mouse,Rat (CL488-60154)

    FC experiment of Jurkat using CL488-60154

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human SMN (Human,Mouse,Rat) (CL488-60154, Clone:3C10B5) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60154 bindet in IF, FC (Intra) SMN (Human,Mouse,Rat) und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen SMN (Human,Mouse,Rat) fusion protein Ag14333
Vollständiger Name survival of motor neuron 2, centromeric
Berechnetes Molekulargewicht 282 aa, 30 kDa
Beobachtetes Molekulargewicht 38 kDa
GenBank-ZugangsnummerBC000908
Gene symbol SMN2
Gene ID (NCBI) 6607
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-2-Ig can recognize human,mouse and rat SMN gene.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 SMN (Human,Mouse,Rat) antibody CL488-60154Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen